Any litter can have multiple sires. It all depends on the timing of mating with the mother. If she bred with several males on the day or days she was able to conceive, and one of those males was part wolf, some of the puppies could be as well. The only way to accurately ascertain the sire of each puppy would be to DNA test the dam and any possible sire, and all puppies. There are various labs in the US that do parentage tests, including Animal Genetics in Florida. There is no DNA test that will tell how much wolf is in a dog though, despite claims there are.
Answered by paws4hisglory - 2/20/2012 6:28:04 PM